[{"id":286,"title":"Hybridization of the Position Sensitive Tunable Laser Diode Microspectroscopy and Laser Ionization Mass Spectrometry on the Scanning Microscopic Stage for Multiparametric Colocalization Imaging","sha":"36f7584a8fdee8e9cd1936d246582735","user_id":null,"state":"published","body":"To date there are known methods for mass-spectrometric control of the surgical samples, including MALDI imaging which applies a laser beam. But in most cases these methods provide nonselective measurements of the samples, which were previously identified and labeled by the morphologists or pathologists. However, a tissue itself is not only a micro-heterogeneous medium, but it is also heterogeneous in the chemical composition of the constituent cells. Chemistry of the single cells and tissue zones is a diagnostic criterion both for determination of the healthy tissue type and for differential diagnostics of the cellular pathologies. Consequently, it is necessary to perform either a position-sensitive sample scanning or microspectrophotometric measurements in situ at the sample holder of the mass-spectrometer. The former cannot be performed within the acceptable time for surgery and express-diagnostics needs, which includes several minutes, while the latter is rather possible and requires a targeted analysis in the ion beam with the subsequent filtration of the excess exposure. We propose to hybridize a trinocular stereoscopic microscope with scanning laser ionization imaging module (MALDI, ELDI, MALDESI) and tunable diode laser spectroscopy module (TDLSM).  ","created_at":"2016-03-04T21:12:16.975Z","updated_at":"2016-03-05T06:00:33.022Z","zenodo_id":100154,"doi":"http://dx.doi.org/10.5281/zenodo.47012","subject":null,"tags":["ms","tdls","maldi","microspectrometer","imaging"],"vote_count":0,"view_count":1,"score":0.1,"deleted":false,"muted":false,"tweeted":true,"attachment_file_name":null,"attachment_content_type":null,"attachment_file_size":null,"attachment_updated_at":null}]